Analysis Note

Positive ControlMouse or rat brain extract or unmyelinated axons

Application

Frozen Sections (1-2 g/ml)Immunoblotting (2-5 g/ml)Immunofluorescence (1-2 g/ml)

General description

Recognizes the ~190 kDa ankyrin_G protein in brain extracts. Reacts with all spliced forms of ankyrin_G.Antibody Target Gene Symbol: ANK3 Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049 Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G) Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057) Mu Entrez ID: 11735 Rat Entrez ID: 361833

Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of Ankyrin_G.

Immunogen

a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin_G

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ankyrin_G is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.

Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.Kunimoto, M., et al. 1995. FEBS Lett.357, 217.Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.Otto, E., et al. 1991. J. Cell Biol.114, 241.Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)